Chicken IgG (H&L) Antibody Biotin Conjugated, Goat, Polyclonal

Artikelnummer: ROC-603-106-002
Artikelname: Chicken IgG (H&L) Antibody Biotin Conjugated, Goat, Polyclonal
Artikelnummer: ROC-603-106-002
Hersteller Artikelnummer: 603-106-002
Alternativnummer: ROC-603-106-002
Hersteller: Rockland Immunochemicals
Wirt: Goat
Kategorie: Antikörper
Applikation: ELISA
Spezies Reaktivität: Gallus
Immunogen: Chicken IgG whole molecule
Konjugation: Biotin
Alternative Synonym: goat anti-Chicken IgG Antibody Biotin Conjugation, goat anti-Chicken IgG BAC Conjugated Antibody, goat anti-Chicken IgY Antibody Biotin Conjugation, goat anti-Chicken IgY BAC Conjugated Antibody
Format: IgG
Spezifität: IgG (H+L)
Minimale Kreuzreaktivität (MinX): no cross-adsorbtion
Klonalität: Polyclonal
Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Chicken
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA: 1:98,500, IHC: 1:500 - 1:3,000, WB: 1:3,000 - 1:30,000
Anwendungsbeschreibung: Anti-Chicken IgG Biotin conjugated antibody has been tested by ELISA and is suitable for ELISA, Western Blot and Immunohistochemistry applications. Specific conditions for reactivity should be optimized by end user.
ELISA results of purified Goat anti-Chicken IgG Biotin Conjugated Antibody tested against purified Chicken IgG (p/n 003-0102). Each well was coated in duplicate with 1.0 µg of Chicken IgY (blue line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Streptavidin HRP conjugate, and TMB-8000 substrate.