Chicken IgG (H&L) Antibody Biotin Conjugated, Goat, Polyclonal

Product Overview

• Article Name: Chicken IgG (H&L) Antibody Biotin Conjugated, Goat, Polyclonal
• Biozol Catalog Number: ROC-603-106-002
• Supplier Catalog Number: 603-106-002
• Alternative Catalog Number: ROC-603-106-002
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA
• Species Reactivity: Gallus
• Immunogen: Chicken IgG whole molecule
• Conjugation: Biotin
• Alternative Names: goat anti-Chicken IgG Antibody Biotin Conjugation, goat anti-Chicken IgG BAC Conjugated Antibody, goat anti-Chicken IgY Antibody Biotin Conjugation, goat anti-Chicken IgY BAC Conjugated Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Chicken
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:98,500, IHC: 1:500 - 1:3,000, WB: 1:3,000 - 1:30,000
• Application Notes: Anti-Chicken IgG Biotin conjugated antibody has been tested by ELISA and is suitable for ELISA, Western Blot and Immunohistochemistry applications. Specific conditions for reactivity should be optimized by end user.

Images

ELISA results of purified Goat anti-Chicken IgG Biotin Conjugated Antibody tested against purified Chicken IgG (p/n 003-0102). Each well was coated in duplicate with 1.0 µg of Chicken IgY (blue line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Streptavidin HRP conjugate, and TMB-8000 substrate.