Rabbit IgG anti-Goat IgG (H+L)-HRPO, MinX none

Artikelnummer: ROC-605-4302
Artikelname: Rabbit IgG anti-Goat IgG (H+L)-HRPO, MinX none
Artikelnummer: ROC-605-4302
Hersteller Artikelnummer: 605-4302
Alternativnummer: ROC-605-4302
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA,IHC,WB
Spezies Reaktivität: Goat
Immunogen: Goat IgG whole molecule
Konjugation: HRPO
Alternative Synonym: rabbit anti-Goat IgG Peroxidase Conjugated Antibody, rabbit anti-Goat IgG HRP Conjugated Antibody, HRP anti-goat IgG, Peroxidase anti-Goat IgG
Format: IgG
Spezifität: IgG (H+L)
Minimale Kreuzreaktivität (MinX): no cross-adsorbtion
Klonalität: Polyclonal
Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Goat
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA: 1:50,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:5,000 - 1:20,000
Anwendungsbeschreibung: Anti-Goat IgG Peroxidase conjugate has been tested by ELISA and western blot. This antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays r
Western Blot of Rabbit anti-GOAT IgG (H&L) Antibody Peroxidase Conjugated. Lane 1: Goat IgG. Load: 100 ng per lane. Secondary antibody: GOAT IgG (H&L) Antibody Peroxidase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT.
ELISA results of Rabbit Anti-Goat IgG Antibody Peroxidase Conjugated tested against purified Goat IgG protein. Each well was coated in duplicate with 1.0 µg of Goat IgG (p/n 005-0102). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. The titer is 1:22,700. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate p/n TMBE-1000.