Rabbit IgG anti-Goat IgG (H+L)-HRPO, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Goat IgG (H+L)-HRPO, MinX none
• Biozol Catalog Number: ROC-605-4302
• Supplier Catalog Number: 605-4302
• Alternative Catalog Number: ROC-605-4302
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Goat
• Immunogen: Goat IgG whole molecule
• Conjugation: HRPO
• Alternative Names: rabbit anti-Goat IgG Peroxidase Conjugated Antibody, rabbit anti-Goat IgG HRP Conjugated Antibody, HRP anti-goat IgG, Peroxidase anti-Goat IgG
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Goat
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:50,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:5,000 - 1:20,000
• Application Notes: Anti-Goat IgG Peroxidase conjugate has been tested by ELISA and western blot. This antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays r

Images

Western Blot of Rabbit anti-GOAT IgG (H&L) Antibody Peroxidase Conjugated. Lane 1: Goat IgG. Load: 100 ng per lane. Secondary antibody: GOAT IgG (H&L) Antibody Peroxidase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT.

ELISA results of Rabbit Anti-Goat IgG Antibody Peroxidase Conjugated tested against purified Goat IgG protein. Each well was coated in duplicate with 1.0 µg of Goat IgG (p/n 005-0102). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. The titer is 1:22,700. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate p/n TMBE-1000.