Rabbit IgG anti-Goat IgG (H+L)-Biotin, MinX none

Produktübersicht

• Artikelname: Rabbit IgG anti-Goat IgG (H+L)-Biotin, MinX none
• Artikelnummer: ROC-605-4602
• Hersteller Artikelnummer: 605-4602
• Alternativnummer: ROC-605-4602
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Goat
• Immunogen: Goat IgG whole molecule
• Konjugation: Biotin
• Alternative Synonym: rabbit Anti-Goat IgG Biotin conjugated antibody, Conjugated Secondary Antibody
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Goat
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Anwendungsbeschreibung: Anti-Goat IgG Biotin Antibody has been tested by ELISA. Secondary antibody reagents are ideal for western blotting, Immunohistochemistry, ELISA, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.

Bilder

ELISA Results of Rabbit Anti-Goat IgG Antibody Biotin Conjugated tested against purified Goat IgG Biotin. Each well was coated in duplicate with 1.0 µg of Goat IgG (p/n 005-0102). The working dilution is 1:13,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076), Streptavidin-HRP conjugated (p/n S000-03) and TMB substrate (p/n TMBE-1000).