Rabbit IgG anti-Goat IgG (H+L)-Biotin, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Goat IgG (H+L)-Biotin, MinX none
• Biozol Catalog Number: ROC-605-4602
• Supplier Catalog Number: 605-4602
• Alternative Catalog Number: ROC-605-4602
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Goat
• Immunogen: Goat IgG whole molecule
• Conjugation: Biotin
• Alternative Names: rabbit Anti-Goat IgG Biotin conjugated antibody, Conjugated Secondary Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Goat
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Goat IgG Biotin Antibody has been tested by ELISA. Secondary antibody reagents are ideal for western blotting, Immunohistochemistry, ELISA, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.

Images

ELISA Results of Rabbit Anti-Goat IgG Antibody Biotin Conjugated tested against purified Goat IgG Biotin. Each well was coated in duplicate with 1.0 µg of Goat IgG (p/n 005-0102). The working dilution is 1:13,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076), Streptavidin-HRP conjugated (p/n S000-03) and TMB substrate (p/n TMBE-1000).