Guinea Pig IgG (H&L) Antibody, Goat, Polyclonal

Artikelnummer: ROC-606-1102
Artikelname: Guinea Pig IgG (H&L) Antibody, Goat, Polyclonal
Artikelnummer: ROC-606-1102
Hersteller Artikelnummer: 606-1102
Alternativnummer: ROC-606-1102
Hersteller: Rockland Immunochemicals
Wirt: Goat
Kategorie: Antikörper
Applikation: ELISA
Spezies Reaktivität: Guinea pig
Immunogen: Guinea Pig IgG whole molecule
Konjugation: Unconjugated
Alternative Synonym: Goat Anti-Guinea Pig IgG, Goat Anti Guinea Pig IgG
Format: IgG
Spezifität: IgG (H+L)
Minimale Kreuzreaktivität (MinX): no cross-adsorbtion
Klonalität: Polyclonal
Konzentration: 2.1 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Liquid (sterile filtered)
Target-Kategorie: Guinea Pig
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA: 1:15,000 - 1:75,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Anwendungsbeschreibung: Anti-Guinea Pig IgG antibody has been tested by ELISA and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
ELISA Results of Goat Anti-Guinea Pig IgG Antibody tested against purified Guinea Pig IgG. Each well was coated in duplicate with 1.0 µg of Guinea Pig IgG (p/n 006-0102). The working dilution is 1:28,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer (p/n MB-076), Donkey Anti-Goat IgG HRP conjugated (p/n 605-703-125), and TMB substrate (p/n TMBE-1000).