Guinea Pig IgG (H&L) Antibody, Goat, Polyclonal

Catalog Number: ROC-606-1102
Article Name: Guinea Pig IgG (H&L) Antibody, Goat, Polyclonal
Biozol Catalog Number: ROC-606-1102
Supplier Catalog Number: 606-1102
Alternative Catalog Number: ROC-606-1102
Manufacturer: Rockland Immunochemicals
Host: Goat
Category: Antikörper
Application: ELISA
Species Reactivity: Guinea pig
Immunogen: Guinea Pig IgG whole molecule
Conjugation: Unconjugated
Alternative Names: Goat Anti-Guinea Pig IgG, Goat Anti Guinea Pig IgG
Format: IgG
Target Specificity: IgG (H+L)
Cross-Adsorption (MinX): no cross-adsorbtion
Clonality: Polyclonal
Concentration: 2.1 mg/mL by UV absorbance at 280 nm
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Liquid (sterile filtered)
Target: Guinea Pig
Antibody Type: Secondary Antibody
Application Dilute: ELISA: 1:15,000 - 1:75,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Application Notes: Anti-Guinea Pig IgG antibody has been tested by ELISA and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
ELISA Results of Goat Anti-Guinea Pig IgG Antibody tested against purified Guinea Pig IgG. Each well was coated in duplicate with 1.0 µg of Guinea Pig IgG (p/n 006-0102). The working dilution is 1:28,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer (p/n MB-076), Donkey Anti-Goat IgG HRP conjugated (p/n 605-703-125), and TMB substrate (p/n TMBE-1000).