Guinea Pig IgG (H&L) Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Guinea Pig IgG (H&L) Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-606-4102
• Hersteller Artikelnummer: 606-4102
• Alternativnummer: ROC-606-4102
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA
• Spezies Reaktivität: Guinea pig
• Immunogen: Guinea Pig IgG whole molecule
• Konjugation: Unconjugated
• Alternative Synonym: Rabbit Anti-Guinea Pig IgG, Rabbit Anti Guinea Pig IgG
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 2.1 mg/mL by UV absorbance at 280 nm
• Puffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Guinea Pig
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:50,000 - 1:125,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Anwendungsbeschreibung: Anti-Guinea Pig IgG antibody has been tested by ELISA and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Bilder

ELISA Results of Purified Rabbit Anti-Guinea Pig IgG Antibody tested against purified Guinea Pig IgG. Each well was coated in duplicate with 10 µg of Guinea Pig IgG (p/n 006-0102). The working dilution is 1:120,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer (p/n MB-066), Goat Anti-Rabbit IgG HRP conjugated (p/n 611-103-122), and TMB substrate (p/n TMBE-1000).