Anti-Guinea Pig IgG antibody has been tested by ELISA and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
ELISA Results of Purified Rabbit Anti-Guinea Pig IgG Antibody tested against purified Guinea Pig IgG. Each well was coated in duplicate with 10 µg of Guinea Pig IgG (p/n 006-0102). The working dilution is 1:120,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer (p/n MB-066), Goat Anti-Rabbit IgG HRP conjugated (p/n 611-103-122), and TMB substrate (p/n TMBE-1000).
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