Rabbit IgG anti-Horse IgG (F(ab)2)-HRPO, MinX none

Produktübersicht

• Artikelname: Rabbit IgG anti-Horse IgG (F(ab)2)-HRPO, MinX none
• Artikelnummer: ROC-608-4304
• Hersteller Artikelnummer: 608-4304
• Alternativnummer: ROC-608-4304
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Equine
• Immunogen: Horse IgG F(ab)2 fragment
• Konjugation: HRPO
• Alternative Synonym: Rabbit Anti-Horse IgG Fab2 Fragment peroxidase Conjugated Antibody, Rabbit Anti-Horse IgG F(ab)2 Antibody peroxidase conjugate, Rabbit Anti-Horse IgG Fab2 Antibody HRP Conjugation
• Format: IgG
• Spezifität: IgG (F(ab')2)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.5 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Horse
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Anwendungsbeschreibung: Anti-Horse IgG F(ab)2 Peroxidase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot

Bilder

ELISA Results of Purified Rabbit Anti-Horse IgG F(ab)2 Antibody Peroxidase Conjugated tested against purified Horse IgG F(ab)2. Each well was coated in duplicate with 10 µg of Horse IgG F(ab)2 (p/n 008-0104). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer (p/n MB-066), Horse IgG F(ab)2 HRP conjugated, and TMB substrate (p/n TMBE-1000).