Rabbit IgG anti-Horse IgG (F(ab)2)-HRPO, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Horse IgG (F(ab)2)-HRPO, MinX none
• Biozol Catalog Number: ROC-608-4304
• Supplier Catalog Number: 608-4304
• Alternative Catalog Number: ROC-608-4304
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Equine
• Immunogen: Horse IgG F(ab)2 fragment
• Conjugation: HRPO
• Alternative Names: Rabbit Anti-Horse IgG Fab2 Fragment peroxidase Conjugated Antibody, Rabbit Anti-Horse IgG F(ab)2 Antibody peroxidase conjugate, Rabbit Anti-Horse IgG Fab2 Antibody HRP Conjugation
• Format: IgG
• Target Specificity: IgG (F(ab')2)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.5 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Horse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Application Notes: Anti-Horse IgG F(ab)2 Peroxidase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot

Images

ELISA Results of Purified Rabbit Anti-Horse IgG F(ab)2 Antibody Peroxidase Conjugated tested against purified Horse IgG F(ab)2. Each well was coated in duplicate with 10 µg of Horse IgG F(ab)2 (p/n 008-0104). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer (p/n MB-066), Horse IgG F(ab)2 HRP conjugated, and TMB substrate (p/n TMBE-1000).