Goat IgG anti-Mouse IgG (Fc)-unconj., MinX none

Produktübersicht

• Artikelname: Goat IgG anti-Mouse IgG (Fc)-unconj., MinX none
• Artikelnummer: ROC-610-1103
• Hersteller Artikelnummer: 610-1103
• Alternativnummer: ROC-610-1103
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Mouse
• Immunogen: Anti-Mouse IgG F(c) fragment was produced by repeated immunization with Mouse IgG F(c) fragment in goat.
• Konjugation: Unconjugated
• Alternative Synonym: goat anti-Mouse IgG F(c) Antibody, Gt-a-Mouse, Mouse IgG Fc Antibody in Goat, Mouse Secondary Antibody
• Format: IgG
• Spezifität: IgG (Fc)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 2.3 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Mouse
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:20,000 - 1:50,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Anwendungsbeschreibung: Anti-Mouse IgG F(c) fragment has been tested by ELISA, dot blot, and wester blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal d

Bilder

ELISA results of Goat anti-Mouse IgG F(c) Antibody tested against purified Mouse IgG F(c) protein. Each well was coated in duplicate with 1.0 µg of Mouse IgG F(c) (p/n 010-0103). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Donkey anti-Goat IgG Antibody Peroxidase Conjugated (Min X Ch GP Ham Hs Ms Rb & Rt Serum Proteins) (p/n 605-703-125) and TMB substrate p/n TMBE-1000.