Goat IgG anti-Mouse IgG (Fc)-unconj., MinX none

Product Overview

• Article Name: Goat IgG anti-Mouse IgG (Fc)-unconj., MinX none
• Biozol Catalog Number: ROC-610-1103
• Supplier Catalog Number: 610-1103
• Alternative Catalog Number: ROC-610-1103
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Anti-Mouse IgG F(c) fragment was produced by repeated immunization with Mouse IgG F(c) fragment in goat.
• Conjugation: Unconjugated
• Alternative Names: goat anti-Mouse IgG F(c) Antibody, Gt-a-Mouse, Mouse IgG Fc Antibody in Goat, Mouse Secondary Antibody
• Format: IgG
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.3 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:50,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Mouse IgG F(c) fragment has been tested by ELISA, dot blot, and wester blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal d

Images

ELISA results of Goat anti-Mouse IgG F(c) Antibody tested against purified Mouse IgG F(c) protein. Each well was coated in duplicate with 1.0 µg of Mouse IgG F(c) (p/n 010-0103). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Donkey anti-Goat IgG Antibody Peroxidase Conjugated (Min X Ch GP Ham Hs Ms Rb & Rt Serum Proteins) (p/n 605-703-125) and TMB substrate p/n TMBE-1000.