Chicken IgG anti-Rabbit IgG (H+L)-FITC, MinX none, Gallus, Polyclonal

Produktübersicht

• Artikelname: Chicken IgG anti-Rabbit IgG (H+L)-FITC, MinX none, Gallus, Polyclonal
• Artikelnummer: ROC-611-902-002
• Hersteller Artikelnummer: 611-902-002
• Alternativnummer: ROC-611-902-002
• Hersteller: Rockland Immunochemicals
• Wirt: Gallus
• Kategorie: Antikörper
• Applikation: FLISA,FACS,IF
• Spezies Reaktivität: Rabbit
• Immunogen: Rabbit IgG whole molecule
• Konjugation: FITC
• Alternative Synonym: chicken anti-Rabbit IgG Antibody fluorescein Conjugation, chicken anti-Rabbit IgG FITC Conjugated Antibody
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Rabbit
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
• Anwendungsbeschreibung: Anti-Rabbit IgG (H&L) Fluorescein Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multipl

Bilder

Dot Blot of Rabbit IgG Antibody Fluorescein Conjugated. Antigen: Rabbit IgG Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng. Primary antibody: none. Secondary antibody: Rabbit IgG Antibody Fluorescein Conjugated 1:1,000 for 60 min at RT. Block: MB-070 for 1 HR at RT.

Western Blot of Anti-Rabbit IgG (H&L) (CHICKEN) Antibody (p/n 611-901-002). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (CHICKEN) Antibody (p/n 611-901-002) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Chicken IgG (GOAT) Peroxidase Conjugated Antibody (p/n 603-103-126) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.