Chicken IgG anti-Rabbit IgG (H+L)-FITC, MinX none, Gallus, Polyclonal

Product Overview

• Article Name: Chicken IgG anti-Rabbit IgG (H+L)-FITC, MinX none, Gallus, Polyclonal
• Biozol Catalog Number: ROC-611-902-002
• Supplier Catalog Number: 611-902-002
• Alternative Catalog Number: ROC-611-902-002
• Manufacturer: Rockland Immunochemicals
• Host: Gallus
• Category: Antikörper
• Application: FLISA,FACS,IF
• Species Reactivity: Rabbit
• Immunogen: Rabbit IgG whole molecule
• Conjugation: FITC
• Alternative Names: chicken anti-Rabbit IgG Antibody fluorescein Conjugation, chicken anti-Rabbit IgG FITC Conjugated Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Rabbit
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
• Application Notes: Anti-Rabbit IgG (H&L) Fluorescein Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multipl

Images

Dot Blot of Rabbit IgG Antibody Fluorescein Conjugated. Antigen: Rabbit IgG Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng. Primary antibody: none. Secondary antibody: Rabbit IgG Antibody Fluorescein Conjugated 1:1,000 for 60 min at RT. Block: MB-070 for 1 HR at RT.

Western Blot of Anti-Rabbit IgG (H&L) (CHICKEN) Antibody (p/n 611-901-002). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (CHICKEN) Antibody (p/n 611-901-002) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Chicken IgG (GOAT) Peroxidase Conjugated Antibody (p/n 603-103-126) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.