Goat IgG anti-Donkey IgG (H+L)-HRPO, MinX none

Produktübersicht

• Artikelname: Goat IgG anti-Donkey IgG (H+L)-HRPO, MinX none
• Artikelnummer: ROC-616-1302
• Hersteller Artikelnummer: 616-1302
• Alternativnummer: ROC-616-1302
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Donkey
• Immunogen: Donkey IgG whole molecule
• Konjugation: HRPO
• Alternative Synonym: goat anti-Donkey IgG Antibody peroxidase conjugation, goat anti-Donkey IgG HRP conjugated Antibody
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Donkey
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:150,000 - 1:250,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:10,000
• Anwendungsbeschreibung: Anti-Donkey IgG Peroxidase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consis

Bilder

ELISA Results of Goat Anti-Donkey IgG Antibody Peroxidase Conjugate tested against purified Donkey IgG HRP. Each well was coated in duplicate with 1.0 µg of Donkey IgG (p/n 016-0102). The working dilution is 1:188,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076) and TMB substrate (p/n TMBE-1000).