Goat IgG anti-Donkey IgG (H+L)-HRPO, MinX none

Product Overview

• Article Name: Goat IgG anti-Donkey IgG (H+L)-HRPO, MinX none
• Biozol Catalog Number: ROC-616-1302
• Supplier Catalog Number: 616-1302
• Alternative Catalog Number: ROC-616-1302
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Donkey
• Immunogen: Donkey IgG whole molecule
• Conjugation: HRPO
• Alternative Names: goat anti-Donkey IgG Antibody peroxidase conjugation, goat anti-Donkey IgG HRP conjugated Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Donkey
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:150,000 - 1:250,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Donkey IgG Peroxidase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consis

Images

ELISA Results of Goat Anti-Donkey IgG Antibody Peroxidase Conjugate tested against purified Donkey IgG HRP. Each well was coated in duplicate with 1.0 µg of Donkey IgG (p/n 016-0102). The working dilution is 1:188,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076) and TMB substrate (p/n TMBE-1000).