F(ab)2 Anti-Chicken IgG Peroxidase Conjugated Antibody has been tested by ELISA and is ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.
ELISA results of purified F(ab)2 anti-Chicken IgG Peroxidase Conjugated Antibody tested against purified chicken IgG. Each well was coated in duplicate with 1.0 µg of chicken IgG/IgY [p/n 003-0102] (purple line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB-1000 substrate.
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