Rabbit F(ab)2 anti-Chicken IgG (H+L)-HRPO, MinX none

Product Overview

• Article Name: Rabbit F(ab)2 anti-Chicken IgG (H+L)-HRPO, MinX none
• Biozol Catalog Number: ROC-703-403-002
• Supplier Catalog Number: 703-403-002
• Alternative Catalog Number: ROC-703-403-002
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Gallus
• Immunogen: Chicken IgG whole molecule
• Conjugation: HRPO
• Alternative Names: Rabbit F(ab)2 Anti-Chicken IgG Peroxidase Conjugated Antibody, Rabbit Fab2 Anti Chicken Peroxidase Conjugated Antibody, Rabbit Fab2 Anti-Chicken IgG Antibody HRP Conjugation, Rabbit F(ab)2 Anti-Chicken IgY Peroxidase, Rabbit F(ab)2 Anti-Chicken IgY HRP
• Format: F(ab')2
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Chicken
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:7,500 - 1:30,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:3,000
• Application Notes: F(ab)2 Anti-Chicken IgG Peroxidase Conjugated Antibody has been tested by ELISA and is ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.

Images

ELISA results of purified F(ab)2 anti-Chicken IgG Peroxidase Conjugated Antibody tested against purified chicken IgG. Each well was coated in duplicate with 1.0 µg of chicken IgG/IgY [p/n 003-0102] (purple line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB-1000 substrate.