Donkey F(ab)2 anti-Rabbit IgG (H+L)-RPE, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Ms,Rt,Sh, Polyclonal

Artikelnummer: ROC-711-708-127
Artikelname: Donkey F(ab)2 anti-Rabbit IgG (H+L)-RPE, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Ms,Rt,Sh, Polyclonal
Artikelnummer: ROC-711-708-127
Hersteller Artikelnummer: 711-708-127
Alternativnummer: ROC-711-708-127
Hersteller: Rockland Immunochemicals
Wirt: Donkey
Kategorie: Antikörper
Applikation: FACS,IHC,IF,WB
Spezies Reaktivität: Rabbit
Immunogen: Fab2 Anti-Rabbit IgG was produced by repeated immunization with Rabbit IgG whole molecule in donkey.
Konjugation: RPE
Alternative Synonym: Donkey F(ab)2 Anti-Rabbit IgG Phycoerythrin Conjugated Antibody, Donkey Fab2 Anti-Rabbit IgG Antibody PE Conjugation
Format: F(ab')2
Spezifität: IgG (H+L)
Minimale Kreuzreaktivität (MinX): Bovine,Gallus,Goat,Guinea pig,Hamster (all),Equine,Human,Mouse,Rat,Sheep
Klonalität: Polyclonal
Konzentration: 0.5 mg/mL by absorbance = 82.0 at 565 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Rabbit
Antibody Type: Secondary Antibody
Application Verdünnung: Flow Cytometry: 1:100 - 1:250, IHC: User Optimized, IF Microscopy: 1:100 - 1:200, WB: 1:1,000
Anwendungsbeschreibung: F(ab)2 Rabbit IgG Phycoerythrin Conjugated Antibody was tested in WB, and is suitable for immunomicroscopy, flow cytometry or FACS analysis, as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) med
Western blot of Phycoerythrin conjugated Donkey F(ab)2 Anti-Rabbit IgG Pre-Adsorbed secondary antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Phycoerythrin donkey secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.