Donkey F(ab)2 anti-Rabbit IgG (H+L)-RPE, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Ms,Rt,Sh, Polyclonal

Product Overview

• Article Name: Donkey F(ab)2 anti-Rabbit IgG (H+L)-RPE, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Ms,Rt,Sh, Polyclonal
• Biozol Catalog Number: ROC-711-708-127
• Supplier Catalog Number: 711-708-127
• Alternative Catalog Number: ROC-711-708-127
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Application: FACS,IHC,IF,WB
• Species Reactivity: Rabbit
• Immunogen: Fab2 Anti-Rabbit IgG was produced by repeated immunization with Rabbit IgG whole molecule in donkey.
• Conjugation: RPE
• Alternative Names: Donkey F(ab)2 Anti-Rabbit IgG Phycoerythrin Conjugated Antibody, Donkey Fab2 Anti-Rabbit IgG Antibody PE Conjugation
• Format: F(ab')2
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Bovine,Gallus,Goat,Guinea pig,Hamster (all),Equine,Human,Mouse,Rat,Sheep

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/mL by absorbance = 82.0 at 565 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Rabbit
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: Flow Cytometry: 1:100 - 1:250, IHC: User Optimized, IF Microscopy: 1:100 - 1:200, WB: 1:1,000
• Application Notes: F(ab)2 Rabbit IgG Phycoerythrin Conjugated Antibody was tested in WB, and is suitable for immunomicroscopy, flow cytometry or FACS analysis, as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) med

Images

Western blot of Phycoerythrin conjugated Donkey F(ab)2 Anti-Rabbit IgG Pre-Adsorbed secondary antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Phycoerythrin donkey secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.