F(ab)2 Swine IgG (H&L) Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal

Artikelnummer: ROC-714-403-002
Artikelname: F(ab)2 Swine IgG (H&L) Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal
Artikelnummer: ROC-714-403-002
Hersteller Artikelnummer: 714-403-002
Alternativnummer: ROC-714-403-002
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA
Spezies Reaktivität: Porcine
Immunogen: Swine IgG whole molecule
Konjugation: HRP
Alternative Synonym: Rabbit F(ab)2 Anti-Swine IgG Antibody Peroxidase Conjugation, Rabbit F(ab)2 Anti-Swine IgG HRP Conjugated Antibody
Format: F(ab')2
Spezifität: IgG (H+L)
Minimale Kreuzreaktivität (MinX): no cross-adsorbtion
Klonalität: Polyclonal
Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Swine
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA: 1:10,000 - 1:100,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:10,000
Anwendungsbeschreibung: F(ab)2 Anti-Swine IgG Peroxidase Conjugated Antibody has been tested by ELISA and is ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.
ELISA Results of Rabbit F(ab)2 Anti-Swine IgG Antibody Peroxidase Conjugated. Each well was coated in duplicate with 10 µg of Swine IgG (p/n 014-0102). The working dilution is 105000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS buffer (p/n MB-066), and TMB substrate (p/n TMBE-1000).