F(ab)2 Swine IgG (H&L) Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal

Product Overview

• Article Name: F(ab)2 Swine IgG (H&L) Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-714-403-002
• Supplier Catalog Number: 714-403-002
• Alternative Catalog Number: ROC-714-403-002
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA
• Species Reactivity: Porcine
• Immunogen: Swine IgG whole molecule
• Conjugation: HRP
• Alternative Names: Rabbit F(ab)2 Anti-Swine IgG Antibody Peroxidase Conjugation, Rabbit F(ab)2 Anti-Swine IgG HRP Conjugated Antibody
• Format: F(ab')2
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Swine
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:100,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:10,000
• Application Notes: F(ab)2 Anti-Swine IgG Peroxidase Conjugated Antibody has been tested by ELISA and is ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.

Images

ELISA Results of Rabbit F(ab)2 Anti-Swine IgG Antibody Peroxidase Conjugated. Each well was coated in duplicate with 10 µg of Swine IgG (p/n 014-0102). The working dilution is 105000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS buffer (p/n MB-066), and TMB substrate (p/n TMBE-1000).