Fab Sheep IgG (H&L) Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal

Artikelnummer: ROC-813-4302
Artikelname: Fab Sheep IgG (H&L) Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal
Artikelnummer: ROC-813-4302
Hersteller Artikelnummer: 813-4302
Alternativnummer: ROC-813-4302
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA
Spezies Reaktivität: Sheep
Immunogen: Sheep IgG whole molecule
Konjugation: HRP
Alternative Synonym: Rabbit Fab Anti-Sheep IgG Antibody Peroxidase Conjugation, Rabbit Fab Anti-Sheep IgG HRP Conjugated Antibody
Format: Fab
Spezifität: IgG (H+L)
Minimale Kreuzreaktivität (MinX): no cross-adsorbtion
Klonalität: Polyclonal
Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Sheep
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
Anwendungsbeschreibung: Fab Anti-Sheep IgG Peroxidase Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays req
ELISA Results of Fab Rabbit Anti-Sheep IgG Antibody Peroxidase Conjugated. Each well was coated in duplicate with 1.0 µg of Sheep IgG (p/n 013-0102). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n TMBE-1000).