Fab Sheep IgG (H&L) Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal

Product Overview

• Article Name: Fab Sheep IgG (H&L) Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-813-4302
• Supplier Catalog Number: 813-4302
• Alternative Catalog Number: ROC-813-4302
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA
• Species Reactivity: Sheep
• Immunogen: Sheep IgG whole molecule
• Conjugation: HRP
• Alternative Names: Rabbit Fab Anti-Sheep IgG Antibody Peroxidase Conjugation, Rabbit Fab Anti-Sheep IgG HRP Conjugated Antibody
• Format: Fab
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Sheep
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Application Notes: Fab Anti-Sheep IgG Peroxidase Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays req

Images

ELISA Results of Fab Rabbit Anti-Sheep IgG Antibody Peroxidase Conjugated. Each well was coated in duplicate with 1.0 µg of Sheep IgG (p/n 013-0102). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n TMBE-1000).