LRRC7 Rabbit pAb, Unconjugated

Artikelnummer: ABB-A13114
Artikelname: LRRC7 Rabbit pAb, Unconjugated
Artikelnummer: ABB-A13114
Hersteller Artikelnummer: A13114
Alternativnummer: ABB-A13114-20UL,ABB-A13114-100UL,ABB-A13114-1000UL,ABB-A13114-500UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IF, WB
Spezies Reaktivität: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: DENSIN, LRRC7
Predicted to be involved in several processes, including establishment or maintenance of epithelial cell apical/basal polarity, positive regulation of neuron projection development, and receptor clustering. Located in several cellular components, including centrosome, cytosol, and nucleoplasm.
Klonalität: Polyclonal
Molekulargewicht: 173kDa
NCBI: 57554
UniProt: Q96NW7
Reinheit: Affinity purification
Sequenz: FPQPLDSKPLLSQREAVPPGNIPQRPDRLPMSDTFTDNWTDGSHYDNTGFVAEETTAENANSNPLLSSKSRSTSSHGRRPLIRQDRIVGVPLELEQSTHRHTPETEVPPSNPWQNWTRTPSPFEDRTAFPSKLETTPTTSPLPERKEHIKESTEIPSPFSP
Target-Kategorie: LRRC7
Antibody Type: Primary Antibody
Application Verdünnung: WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Signal Transduction,Cell Biology Developmental Biology,Cytoskeleton,Intermediate Filaments,Neuroscience, Cell Type Marker,Neuron marker,Synapse marker.
Immunofluorescence analysis of C6 cells using LRRC7 Rabbit pAb (A13114) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using LRRC7 Rabbit pAb (A13114) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western blot analysis of various lysates using LRRC7 Rabbit pAb (A13114) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunofluorescence analysis of U-2 OS cells using LRRC7 Rabbit pAb (A13114) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.