SARS-CoV-2 N Protein Mouse mAb

Produktübersicht

• Artikelname: SARS-CoV-2 N Protein Mouse mAb
• Artikelnummer: ABB-A20142
• Hersteller Artikelnummer: A20142
• Alternativnummer: ABB-A20142-100UL, ABB-A20142-20UL
• Hersteller: ABclonal
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, IF, WB
• Spezies Reaktivität: Virus
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Alternative Synonym: Nucleoprotein, NP, SARS-CoV-2 N Protein, sars-cov-2

Produktbeschreibung

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host SMAD3.

Produkteigenschaften

• Molekulargewicht: 46kDa
• NCBI: 43740575
• UniProt: P59595
• Reinheit: Affinity purification
• Target-Kategorie: Nucleoprotein

Anwendungsbeschreibung

• Application Verdünnung: WB,1:1000 - 1:6000|IF/ICC,1:50 - 1:200|ELISA,1:30000 - 1:120000
• Anwendungsbeschreibung: Cross-Reactivity: SARS-CoV-2

Bilder

Western blot analysis of from wild type (WT) and 293T cells transfected with SARS-CoV-2 N Protein using SARS-CoV-2 N Protein Mouse mAb (A20142) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 20µg/10µg/5µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Immunofluorescence analysis of 293T transfected with SARS-CoV-2 N Protein using SARS-CoV-2 Nucleoprotein mAb mAb (A20142) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Mouse IgG (H+L) (AS008) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immobilized Recombinant 2019-nCoV Nucleocapsid Protein (RP01264LQ) at 1µg/mL (100µL/well) can bind SARS-CoV-2 Nucleoprotein Rabbit mAb (A20142) with a linear range of 3.12-200ng/mL.