SARS-CoV-2 N Protein Mouse mAb

Product Overview

• Article Name: SARS-CoV-2 N Protein Mouse mAb
• Biozol Catalog Number: ABB-A20142
• Supplier Catalog Number: A20142
• Alternative Catalog Number: ABB-A20142-100UL, ABB-A20142-20UL
• Manufacturer: ABclonal
• Host: Mouse
• Category: Antikörper
• Application: ELISA, IF, WB
• Species Reactivity: Virus
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Alternative Names: Nucleoprotein, NP, SARS-CoV-2 N Protein, sars-cov-2

Product Description

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host SMAD3.

Product Properties

• Molecular Weight: 46kDa
• NCBI: 43740575
• UniProt: P59595
• Purity: Affinity purification
• Target: Nucleoprotein

Application Notes

• Application Dilute: WB,1:1000 - 1:6000|IF/ICC,1:50 - 1:200|ELISA,1:30000 - 1:120000
• Application Notes: Cross-Reactivity: SARS-CoV-2

Images

Western blot analysis of from wild type (WT) and 293T cells transfected with SARS-CoV-2 N Protein using SARS-CoV-2 N Protein Mouse mAb (A20142) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 20µg/10µg/5µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Immunofluorescence analysis of 293T transfected with SARS-CoV-2 N Protein using SARS-CoV-2 Nucleoprotein mAb mAb (A20142) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Mouse IgG (H+L) (AS008) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immobilized Recombinant 2019-nCoV Nucleocapsid Protein (RP01264LQ) at 1µg/mL (100µL/well) can bind SARS-CoV-2 Nucleoprotein Rabbit mAb (A20142) with a linear range of 3.12-200ng/mL.