[KD Validated] beta 2 Microglobulin Rabbit mAb, Unconjugated

Produktübersicht

• Artikelname: [KD Validated] beta 2 Microglobulin Rabbit mAb, Unconjugated
• Artikelnummer: ABB-A23430
• Hersteller Artikelnummer: A23430
• Alternativnummer: ABB-A23430-100UL,ABB-A23430-20UL,ABB-A23430-1000UL,ABB-A23430-500UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, FC, IF, IHC-P, WB
• Spezies Reaktivität: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: B2M, IMD43, beta-2-microglobulin, [KD Validated] beta 2 Microglobulin

Produktbeschreibung

This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [ARC60950]
• Molekulargewicht: 13kDa
• NCBI: 567
• UniProt: P61769
• Reinheit: Affinity purification
• Sequenz: IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM
• Target-Kategorie: B2M
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB,1:1000 - 1:4000|IHC-P,1:1000 - 1:4000|IF/ICC,1:50 - 1:200|FC,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human, ResearchArea: Cancer,Tumor biomarkers,Cardiovascular,Blood,Blood Cell Antigens,Serum Proteins.

Bilder

Western blot analysis of lysates from wild type(WT) and beta2-microglobulin knockdown (KD) HeLa cells, using [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Western blot analysis of various lysates, using [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunofluorescence analysis of HeLa cells using [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Flow cytometry:1X10 6 Daudi cells(negative control,left) and Hela cells (right) were surface-stained with [KD Validated] beta Microglobulin Rabbit mAb(A23430,2 µg/mL,orange line) or ABflo 647 Rabbit IgG isotype control (A22070,2 µg/mL,blue line).Non-fluorescently stained cells was used as blank control (red line).

Flow cytometry:1X10 6 HeLa cells were surface-stained with ABflo 647 Rabbit IgG isotype control (A22070,2 µg/mL,left) or beta2 Microglobulin Rabbit mAb(A23430,2 µg/mL,right).