[KD Validated] beta 2 Microglobulin Rabbit mAb, Unconjugated

Product Overview

• Article Name: [KD Validated] beta 2 Microglobulin Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-A23430
• Supplier Catalog Number: A23430
• Alternative Catalog Number: ABB-A23430-100UL,ABB-A23430-20UL,ABB-A23430-1000UL,ABB-A23430-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, FC, IF, IHC-P, WB
• Species Reactivity: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: B2M, IMD43, beta-2-microglobulin, [KD Validated] beta 2 Microglobulin

Product Description

This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ARC60950]
• Molecular Weight: 13kDa
• NCBI: 567
• UniProt: P61769
• Purity: Affinity purification
• Sequence: IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM
• Target: B2M
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:1000 - 1:4000|IHC-P,1:1000 - 1:4000|IF/ICC,1:50 - 1:200|FC,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Cancer,Tumor biomarkers,Cardiovascular,Blood,Blood Cell Antigens,Serum Proteins.

Images

Western blot analysis of lysates from wild type(WT) and beta2-microglobulin knockdown (KD) HeLa cells, using [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Western blot analysis of various lysates, using [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunofluorescence analysis of HeLa cells using [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Flow cytometry:1X10 6 Daudi cells(negative control,left) and Hela cells (right) were surface-stained with [KD Validated] beta Microglobulin Rabbit mAb(A23430,2 µg/mL,orange line) or ABflo 647 Rabbit IgG isotype control (A22070,2 µg/mL,blue line).Non-fluorescently stained cells was used as blank control (red line).

Flow cytometry:1X10 6 HeLa cells were surface-stained with ABflo 647 Rabbit IgG isotype control (A22070,2 µg/mL,left) or beta2 Microglobulin Rabbit mAb(A23430,2 µg/mL,right).