Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation:
Unconjugated
Alternative Synonym:
Hobit,ZNF683
Enables RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Involved in T cell receptor signaling pathway, cellular response to ionomycin, and regulation of gene expression. Predicted to be located in nucleus.
WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung:
Cross-Reactivity: Human. ResearchArea: Epigenetics and Nuclear Signaling Transcription Domain Families Zinc FingerEpigenetics and Nuclear Signaling DNA / RNA RNA Processing OtherEpigenetics and Nuclear Signaling Chromatin Binding Proteins DNA / RNA binding. Shipping: Ice Bag
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with ZNF683 using ZNF683 Rabbit pAb (A26154) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 20 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020) .Exposure time: 10s.
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