PAP Rabbit pAb, Unconjugated, Polyclonal

Artikelnummer: ABB-A27169
Artikelname: PAP Rabbit pAb, Unconjugated, Polyclonal
Artikelnummer: ABB-A27169
Hersteller Artikelnummer: A27169
Alternativnummer: ABB-A27169-100UL,ABB-A27169-500UL,ABB-A27169-1000UL,ABB-A27169-20UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: PAP, PAP-alpha
The protein encoded by this gene belongs to the poly(A) polymerase family. It is required for the addition of adenosine residues for the creation of the 3-poly(A) tail of mRNAs. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Klonalität: Polyclonal
Molekulargewicht: 83kDa
NCBI: 10914
UniProt: P51003
Reinheit: Affinity purification
Sequenz: SPPPKPTVSRVVSSTRLVNPPPRSSGNAATSGNAATKIPTPIVGVKRTSSPHKEESPKKTKTEEDETSEDANCLALSGHDKTEAKEQLDTETSTTQSETIQTAASLLASQKTSSTDLSDIPALPANPIPVIKNSIKLRLNR
Target-Kategorie: PAPOLA
Application Verdünnung: WB,1:3000-1:12000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human. ResearchArea: Tags & Cell Markers Cell Type Markers Tumor AssociatedCell Biology Proteolysis / Ubiquitin Proteolytic enzymes Serine protease KallikreinsCancer Tumor biomarkers Enzymes Phosphatases. Shipping: Ice Bag
Western blot analysis of lysates from HeLa cells using PAP Rabbit pAb (A27169) at 1:3000 dilution incubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.