PAP Rabbit pAb, Unconjugated, Polyclonal

Product Overview

• Article Name: PAP Rabbit pAb, Unconjugated, Polyclonal
• Biozol Catalog Number: ABB-A27169
• Supplier Catalog Number: A27169
• Alternative Catalog Number: ABB-A27169-100UL,ABB-A27169-500UL,ABB-A27169-1000UL,ABB-A27169-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: PAP, PAP-alpha

Product Description

The protein encoded by this gene belongs to the poly(A) polymerase family. It is required for the addition of adenosine residues for the creation of the 3-poly(A) tail of mRNAs. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 83kDa
• NCBI: 10914
• UniProt: P51003
• Purity: Affinity purification
• Sequence: SPPPKPTVSRVVSSTRLVNPPPRSSGNAATSGNAATKIPTPIVGVKRTSSPHKEESPKKTKTEEDETSEDANCLALSGHDKTEAKEQLDTETSTTQSETIQTAASLLASQKTSSTDLSDIPALPANPIPVIKNSIKLRLNR
• Target: PAPOLA

Application Notes

• Application Dilute: WB,1:3000-1:12000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human. ResearchArea: Tags & Cell Markers Cell Type Markers Tumor AssociatedCell Biology Proteolysis / Ubiquitin Proteolytic enzymes Serine protease KallikreinsCancer Tumor biomarkers Enzymes Phosphatases. Shipping: Ice Bag

Images

Western blot analysis of lysates from HeLa cells using PAP Rabbit pAb (A27169) at 1:3000 dilution incubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.