Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation:
Unconjugated
Alternative Synonym:
sf, JM2, scurfin
The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene result in the scurfy phenotype (sf). Alternative splicing results in multiple transcript variants.
WB,1:3000 - 1:18000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung:
Cross-Reactivity: Mouse. ResearchArea: Cell Biology Cell Cycle Cell Division Chromatid CohesionEpigenetics and Nuclear Signaling Transcription Domain Families Forkhead Box FOXPImmunology Adaptive Immunity Regulatory T Cells. Shipping: Ice Bag
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with FOXP3 using FOXP3 Rabbit pAb (A27170) at 1:3000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 20 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020) .Exposure time: 0.5s.
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