PERK Rabbit mAb, Unconjugated, Monoclonal

Produktübersicht

• Artikelname: PERK Rabbit mAb, Unconjugated, Monoclonal
• Artikelnummer: ABB-A27243
• Hersteller Artikelnummer: A27243
• Alternativnummer: ABB-A27243-20UL,ABB-A27243-100UL,ABB-A27243-500UL,ABB-A27243-1000UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: PEK, WRS, PERK

Produktbeschreibung

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome.

Produkteigenschaften

• Klonalität: Monoclonal
• Molekulargewicht: 125kDa
• NCBI: 9451
• UniProt: Q9NZJ5
• Reinheit: Affinity purification
• Target-Kategorie: EIF2AK3

Anwendungsbeschreibung

• Application Verdünnung: WB,1:1000 - 1:4000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Translation Control,Signal Transduction,Kinase,Serine threonine kinases,Cell Biology Developmental Biology,Apoptosis,Endocrine Metabolism,Endocrine and metabolic diseases,Diabetes,Obesity,Neuroscience,Neurodegenerative Diseases. Shipping: Ice Bag

Bilder

Western blot analysis of various lysates using PERK Rabbit mAb (A27243) at 1:1000 dilution incubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.