PERK Rabbit mAb, Unconjugated, Monoclonal

Product Overview

• Article Name: PERK Rabbit mAb, Unconjugated, Monoclonal
• Biozol Catalog Number: ABB-A27243
• Supplier Catalog Number: A27243
• Alternative Catalog Number: ABB-A27243-20UL,ABB-A27243-100UL,ABB-A27243-500UL,ABB-A27243-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: PEK, WRS, PERK

Product Description

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome.

Product Properties

• Clonality: Monoclonal
• Molecular Weight: 125kDa
• NCBI: 9451
• UniProt: Q9NZJ5
• Purity: Affinity purification
• Target: EIF2AK3

Application Notes

• Application Dilute: WB,1:1000 - 1:4000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Translation Control,Signal Transduction,Kinase,Serine threonine kinases,Cell Biology Developmental Biology,Apoptosis,Endocrine Metabolism,Endocrine and metabolic diseases,Diabetes,Obesity,Neuroscience,Neurodegenerative Diseases. Shipping: Ice Bag

Images

Western blot analysis of various lysates using PERK Rabbit mAb (A27243) at 1:1000 dilution incubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.