F4/80 Rabbit mAb, Unconjugated

Produktübersicht

• Artikelname: F4/80 Rabbit mAb, Unconjugated
• Artikelnummer: ABB-A27257
• Hersteller Artikelnummer: A27257
• Alternativnummer: ABB-A27257-1000UL,ABB-A27257-500UL,ABB-A27257-100UL,ABB-A27257-20UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC-P, WB
• Spezies Reaktivität: Mouse
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: Emr1, Ly71, F4/80, Gpf480, TM7LN3, DD7A5-7, EGF-TM7

Produktbeschreibung

Predicted to enable G protein-coupled receptor activity. Predicted to be involved in adenylate cyclase-activating G protein-coupled receptor signaling pathway. Predicted to act upstream of or within G protein-coupled receptor signaling pathway and adaptive immune response. Located in external side of plasma membrane. Is expressed in several structures, including cardiovascular system, central nervous system, genitourinary system, hemolymphoid system, and intestine. Orthologous to human ADGRE1 (adhesion G protein-coupled receptor E1).

Produkteigenschaften

• Molekulargewicht: 102kDa
• NCBI: 13733
• UniProt: Q61549
• Reinheit: Affinity purification
• Target-Kategorie: Adgre1

Anwendungsbeschreibung

• Application Verdünnung: WB,1:1000 - 1:2000|IF-P,1:50 - 1:200|IHC-P,1:200 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Mouse,Rat, ResearchArea: Immunology Innate Immunity Macrophage / Inflamm.Tags & Cell Markers Cell Type Markers Other Cell Types

Bilder

Western blot analysis of various lysates using F4/80 Rabbit mAb (A27257) at 1:1000 dilutionincubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): L929
Exposure time: 45s.

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using F4/80 Rabbit mAb (A27257) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using F4/80 Rabbit mAb (A27257) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using F4/80 Rabbit mAb (A27257) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using F4/80 Rabbit mAb (A27257) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using F4/80 Rabbit mAb (A27257) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using F4/80 Rabbit mAb (A27257) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Confocal imaging of paraffin-embedded Rat spleen tissue using F4/80 Rabbit mAb (A27257, dilution 1:50) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Confocal imaging of paraffin-embedded Mouse spleen tissue using F4/80 Rabbit mAb (A27257, dilution 1:50) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.