F4/80 Rabbit mAb, Unconjugated

Product Overview

• Article Name: F4/80 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-A27257
• Supplier Catalog Number: A27257
• Alternative Catalog Number: ABB-A27257-1000UL,ABB-A27257-500UL,ABB-A27257-100UL,ABB-A27257-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC-P, WB
• Species Reactivity: Mouse
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: Emr1, Ly71, F4/80, Gpf480, TM7LN3, DD7A5-7, EGF-TM7

Product Description

Predicted to enable G protein-coupled receptor activity. Predicted to be involved in adenylate cyclase-activating G protein-coupled receptor signaling pathway. Predicted to act upstream of or within G protein-coupled receptor signaling pathway and adaptive immune response. Located in external side of plasma membrane. Is expressed in several structures, including cardiovascular system, central nervous system, genitourinary system, hemolymphoid system, and intestine. Orthologous to human ADGRE1 (adhesion G protein-coupled receptor E1).

Product Properties

• Molecular Weight: 102kDa
• NCBI: 13733
• UniProt: Q61549
• Purity: Affinity purification
• Target: Adgre1

Application Notes

• Application Dilute: WB,1:1000 - 1:2000|IF-P,1:50 - 1:200|IHC-P,1:200 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Mouse,Rat, ResearchArea: Immunology Innate Immunity Macrophage / Inflamm.Tags & Cell Markers Cell Type Markers Other Cell Types

Images

Western blot analysis of various lysates using F4/80 Rabbit mAb (A27257) at 1:1000 dilutionincubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): L929
Exposure time: 45s.

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using F4/80 Rabbit mAb (A27257) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using F4/80 Rabbit mAb (A27257) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using F4/80 Rabbit mAb (A27257) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using F4/80 Rabbit mAb (A27257) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using F4/80 Rabbit mAb (A27257) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using F4/80 Rabbit mAb (A27257) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Confocal imaging of paraffin-embedded Rat spleen tissue using F4/80 Rabbit mAb (A27257, dilution 1:50) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Confocal imaging of paraffin-embedded Mouse spleen tissue using F4/80 Rabbit mAb (A27257, dilution 1:50) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.