MELK Rabbit mAb, Unconjugated, Monoclonal

Artikelnummer: ABB-A3530
Artikelname: MELK Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer: ABB-A3530
Hersteller Artikelnummer: A3530
Alternativnummer: ABB-A3530-100UL,ABB-A3530-20UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: HPK38, MELK
Enables calcium ion binding activity, non-membrane spanning protein tyrosine kinase activity, and protein serine/threonine kinase activity. Involved in apoptotic process, cell population proliferation, and protein autophosphorylation. Located in cell cortex and plasma membrane.
Klonalität: Monoclonal
Molekulargewicht: 75kDa
NCBI: 9833
UniProt: Q14680
Reinheit: Affinity purification
Target-Kategorie: MELK
Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Signal Transduction,Kinase,Cell Biology Developmental Biology,Cell Cycle,Stem Cells,Embryonic Stem Cells. Shipping: Ice Bag
Western blot analysis of various lysates using MELK Rabbit mAb (A3530) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.