Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation:
Unconjugated
Alternative Names:
HPK38, MELK
Enables calcium ion binding activity, non-membrane spanning protein tyrosine kinase activity, and protein serine/threonine kinase activity. Involved in apoptotic process, cell population proliferation, and protein autophosphorylation. Located in cell cortex and plasma membrane.
WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:
Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Signal Transduction,Kinase,Cell Biology Developmental Biology,Cell Cycle,Stem Cells,Embryonic Stem Cells. Shipping: Ice Bag
Western blot analysis of various lysates using MELK Rabbit mAb (A3530) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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