MELK Rabbit mAb, Unconjugated, Monoclonal

Catalog Number: ABB-A3530
Article Name: MELK Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number: ABB-A3530
Supplier Catalog Number: A3530
Alternative Catalog Number: ABB-A3530-100UL,ABB-A3530-20UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Alternative Names: HPK38, MELK
Enables calcium ion binding activity, non-membrane spanning protein tyrosine kinase activity, and protein serine/threonine kinase activity. Involved in apoptotic process, cell population proliferation, and protein autophosphorylation. Located in cell cortex and plasma membrane.
Clonality: Monoclonal
Molecular Weight: 75kDa
NCBI: 9833
UniProt: Q14680
Purity: Affinity purification
Target: MELK
Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Signal Transduction,Kinase,Cell Biology Developmental Biology,Cell Cycle,Stem Cells,Embryonic Stem Cells. Shipping: Ice Bag
Western blot analysis of various lysates using MELK Rabbit mAb (A3530) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.