Rig-I/DDX58 Rabbit mAb, Unconjugated, Monoclonal

Produktübersicht

• Artikelname: Rig-I/DDX58 Rabbit mAb, Unconjugated, Monoclonal
• Artikelnummer: ABB-A3689
• Hersteller Artikelnummer: A3689
• Alternativnummer: ABB-A3689-100UL,ABB-A3689-20UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: RIG1, DDX58, RIG-I, RLR-1, SGMRT2, Rig-I/DDX58

Produktbeschreibung

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of the antiviral innate immune response. Mutations in this gene are associated with Singleton-Merten syndrome 2.

Produkteigenschaften

• Klonalität: Monoclonal
• Molekulargewicht: 107kDa
• NCBI: 23586
• UniProt: O95786
• Reinheit: Affinity purification
• Target-Kategorie: RIGI

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human. ResearchArea: Epigenetics Nuclear Signaling,Immunology Inflammation,Toll-like Receptor Signaling Pathway. Shipping: Ice Bag

Bilder

Western blot analysis of various lysates, using Rig-I/DDX58 Rabbit mAb (A3689) at 1:1000 dilution. THP-1 cells were treated with LPS (1 µg/ml) at 37°C for 8 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.