Rig-I/DDX58 Rabbit mAb, Unconjugated, Monoclonal

Product Overview

• Article Name: Rig-I/DDX58 Rabbit mAb, Unconjugated, Monoclonal
• Biozol Catalog Number: ABB-A3689
• Supplier Catalog Number: A3689
• Alternative Catalog Number: ABB-A3689-100UL,ABB-A3689-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: RIG1, DDX58, RIG-I, RLR-1, SGMRT2, Rig-I/DDX58

Product Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of the antiviral innate immune response. Mutations in this gene are associated with Singleton-Merten syndrome 2.

Product Properties

• Clonality: Monoclonal
• Molecular Weight: 107kDa
• NCBI: 23586
• UniProt: O95786
• Purity: Affinity purification
• Target: RIGI

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human. ResearchArea: Epigenetics Nuclear Signaling,Immunology Inflammation,Toll-like Receptor Signaling Pathway. Shipping: Ice Bag

Images

Western blot analysis of various lysates, using Rig-I/DDX58 Rabbit mAb (A3689) at 1:1000 dilution. THP-1 cells were treated with LPS (1 µg/ml) at 37°C for 8 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.