ACAT1 Rabbit pAb, Unconjugated, Polyclonal

Artikelnummer: ABB-A5335
Artikelname: ACAT1 Rabbit pAb, Unconjugated, Polyclonal
Artikelnummer: ABB-A5335
Hersteller Artikelnummer: A5335
Alternativnummer: ABB-A5335-100UL,ABB-A5335-20UL,ABB-A5335-500UL,ABB-A5335-1000UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: T2, MAT, ACAT, THIL, ACAT1
This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.
Klonalität: Polyclonal
Molekulargewicht: 45kDa
NCBI: 38
UniProt: P24752
Reinheit: Affinity purification
Sequenz: MAVLAALLRSGARSRSPLLRRLVQEIRYVERSYVSKPTLKEVVIVSATRTPIGSFLGSLSLLPATKLGSIAIQGAIEKAGIPKEEVKEAYMGNVLQGGEGQAPTRQAVLGAGLPISTPCTTINKVCASGMKAIMMASQSLMCGHQ
Target-Kategorie: ACAT1
Antibody Type: Primary Antibody
Application Verdünnung: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Cancer,Signal Transduction,Endocrine Metabolism,Mitochondrial metabolism,Mitochondrial markers,Amino acid metabolism,Lipid Metabolism. Shipping: Ice Bag
Western blot analysis of various lysates, using ACAT1 Rabbit pAb (A5335) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.