ACAT1 Rabbit pAb, Unconjugated, Polyclonal

Catalog Number: ABB-A5335
Article Name: ACAT1 Rabbit pAb, Unconjugated, Polyclonal
Biozol Catalog Number: ABB-A5335
Supplier Catalog Number: A5335
Alternative Catalog Number: ABB-A5335-100UL,ABB-A5335-20UL,ABB-A5335-500UL,ABB-A5335-1000UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Alternative Names: T2, MAT, ACAT, THIL, ACAT1
This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.
Clonality: Polyclonal
Molecular Weight: 45kDa
NCBI: 38
UniProt: P24752
Purity: Affinity purification
Sequence: MAVLAALLRSGARSRSPLLRRLVQEIRYVERSYVSKPTLKEVVIVSATRTPIGSFLGSLSLLPATKLGSIAIQGAIEKAGIPKEEVKEAYMGNVLQGGEGQAPTRQAVLGAGLPISTPCTTINKVCASGMKAIMMASQSLMCGHQ
Target: ACAT1
Antibody Type: Primary Antibody
Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Cancer,Signal Transduction,Endocrine Metabolism,Mitochondrial metabolism,Mitochondrial markers,Amino acid metabolism,Lipid Metabolism. Shipping: Ice Bag
Western blot analysis of various lysates, using ACAT1 Rabbit pAb (A5335) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.