HIRA Rabbit mAb, Unconjugated

Produktübersicht

• Artikelname: HIRA Rabbit mAb, Unconjugated
• Artikelnummer: ABB-A8892
• Hersteller Artikelnummer: A8892
• Alternativnummer: ABB-A8892-20UL, ABB-A8892-100UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: TUP1, DGCR1, TUPLE1, HIRA

Produktbeschreibung

This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.

Produkteigenschaften

• Molekulargewicht: 112kDa
• NCBI: 7290
• UniProt: P54198
• Reinheit: Affinity purification
• Target-Kategorie: HIRA

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Chromatin Remodeling,Cell Biology Developmental Biology,Stem Cells,Germline Stem Cells.

Bilder

Western blot analysis of various lysates using HIRA Rabbit mAb (A8892) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.