HIRA Rabbit mAb, Unconjugated

Product Overview

• Article Name: HIRA Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-A8892
• Supplier Catalog Number: A8892
• Alternative Catalog Number: ABB-A8892-20UL, ABB-A8892-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: TUP1, DGCR1, TUPLE1, HIRA

Product Description

This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.

Product Properties

• Molecular Weight: 112kDa
• NCBI: 7290
• UniProt: P54198
• Purity: Affinity purification
• Target: HIRA

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Chromatin Remodeling,Cell Biology Developmental Biology,Stem Cells,Germline Stem Cells.

Images

Western blot analysis of various lysates using HIRA Rabbit mAb (A8892) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.