Pyruvate carboxylase (PC) Rabbit mAb, Unconjugated

Produktübersicht

• Artikelname: Pyruvate carboxylase (PC) Rabbit mAb, Unconjugated
• Artikelnummer: ABB-A8980
• Hersteller Artikelnummer: A8980
• Alternativnummer: ABB-A8980-20UL, ABB-A8980-100UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: PCB, PC, Pyruvate carboxylase (PC)

Produktbeschreibung

This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5 UTRs, but encoding the same protein, have been found for this gene.

Produkteigenschaften

• Molekulargewicht: 130kDa
• NCBI: 5091
• UniProt: P11498
• Reinheit: Affinity purification
• Target-Kategorie: PC

Anwendungsbeschreibung

• Application Verdünnung: WB,1:1000 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Mouse,Rat, ResearchArea: Cancer,Signal Transduction,Endocrine Metabolism,Lipid Metabolism,Carbohydrate metabolism.

Bilder

Western blot analysis of various lysates using Pyruvate carboxylase (PC) Rabbit mAb (A8980) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.