Pyruvate carboxylase (PC) Rabbit mAb, Unconjugated

Product Overview

• Article Name: Pyruvate carboxylase (PC) Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-A8980
• Supplier Catalog Number: A8980
• Alternative Catalog Number: ABB-A8980-20UL, ABB-A8980-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: PCB, PC, Pyruvate carboxylase (PC)

Product Description

This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5 UTRs, but encoding the same protein, have been found for this gene.

Product Properties

• Molecular Weight: 130kDa
• NCBI: 5091
• UniProt: P11498
• Purity: Affinity purification
• Target: PC

Application Notes

• Application Dilute: WB,1:1000 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Mouse,Rat, ResearchArea: Cancer,Signal Transduction,Endocrine Metabolism,Lipid Metabolism,Carbohydrate metabolism.

Images

Western blot analysis of various lysates using Pyruvate carboxylase (PC) Rabbit mAb (A8980) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.