PPM1E Rabbit mAb, Unconjugated

Artikelnummer: ABB-A9374
Artikelname: PPM1E Rabbit mAb, Unconjugated
Artikelnummer: ABB-A9374
Hersteller Artikelnummer: A9374
Alternativnummer: ABB-A9374-20UL, ABB-A9374-100UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: POPX1, PP2CH, caMKN, CAMKPN, CaMKP-N, PPM1E
This gene encodes a member of the PPM family of serine/threonine-protein phosphatases. The encoded protein is localized to the nucleus and dephosphorylates and inactivates multiple substrates including serine/threonine-protein kinase PAK 1, 5-AMP-activated protein kinase (AMPK) and the multifunctional calcium/calmodulin-dependent protein kinases. Alternatively spliced transcript variants have been observed for this gene.
Molekulargewicht: 84kDa
NCBI: 22843
UniProt: Q8WY54
Reinheit: Affinity purification
Target-Kategorie: PPM1E
Application Verdünnung: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human, ResearchArea: Signal Transduction,Kinase,Cell Biology Developmental Biology,Cell Cycle,Cytoskeleton,Microfilaments.
Western blot analysis of various lysates using PPM1E Rabbit mAb (A9374) at 1:1000 dilution incubated overnight at 4C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180 s.